6SB0

cryo-EM structure of mTORC1 bound to PRAS40-fused active RagA/C GTPases

6SB0 の概要

• エントリーDOI: 10.2210/pdb6sb0/pdb
• EMDBエントリー: 10132
• 分子名称: mTOR,Serine/threonine-protein kinase mTOR,mTOR,Serine/threonine-protein kinase mTOR, Target of rapamycin complex subunit LST8, Ras-related GTP-binding protein A, ... (8 entities in total)
• 機能のキーワード: small gtpases, mtorc1 activator, roadblock domain, gtpase domain, signaling protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 1162771.68

構造登録者

Anandapadamanaban, M.,Berndt, A.,Masson, G.R.,Perisic, O.,Williams, R.L. (登録日: 2019-07-18, 公開日: 2019-10-16, 最終更新日: 2024-05-22)

主引用文献

Anandapadamanaban, M.,Masson, G.R.,Perisic, O.,Berndt, A.,Kaufman, J.,Johnson, C.M.,Santhanam, B.,Rogala, K.B.,Sabatini, D.M.,Williams, R.L.
Architecture of human Rag GTPase heterodimers and their complex with mTORC1.
Science, 366:203-210, 2019

PubMed Abstract: The Rag guanosine triphosphatases (GTPases) recruit the master kinase mTORC1 to lysosomes to regulate cell growth and proliferation in response to amino acid availability. The nucleotide state of Rag heterodimers is critical for their association with mTORC1. Our cryo-electron microscopy structure of RagA/RagC in complex with mTORC1 shows the details of RagA/RagC binding to the RAPTOR subunit of mTORC1 and explains why only the RagA/RagC nucleotide state binds mTORC1. Previous kinetic studies suggested that GTP binding to one Rag locks the heterodimer to prevent GTP binding to the other. Our crystal structures and dynamics of RagA/RagC show the mechanism for this locking and explain how oncogenic hotspot mutations disrupt this process. In contrast to allosteric activation by RHEB, Rag heterodimer binding does not change mTORC1 conformation and activates mTORC1 by targeting it to lysosomes.

PubMed: 31601764
DOI: 10.1126/science.aax3939

実験手法

ELECTRON MICROSCOPY (5.5 Å)