6S8X

Crystal structure of the Rab-binding domain of FIP2

6S8X の概要

• エントリーDOI: 10.2210/pdb6s8x/pdb
• 分子名称: Rab11 family-interacting protein 2, HEXANE-1,6-DIOL (3 entities in total)
• 機能のキーワード: membrane trafficking, rab small gtpases, effector protein, rab-binding domain, endosomal trafficking, signaling protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 36603.43

構造登録者

Kearney, A.M.,Khan, A.R. (登録日: 2019-07-10, 公開日: 2020-08-05, 最終更新日: 2024-05-15)

主引用文献

Kearney, A.M.,Khan, A.R.
Crystal structure of the Rab-binding domain of Rab11 family-interacting protein 2.
Acta Crystallogr.,Sect.F, 76:357-363, 2020

PubMed Abstract: The small GTPases Rab11, Rab14 and Rab25 regulate membrane trafficking through the recruitment of Rab11 family-interacting proteins (FIPs) to endocytic compartments. FIPs are multi-domain effector proteins that have a highly conserved Rab-binding domain (RBD) at their C-termini. Several structures of complexes of Rab11 with RBDs have previously been determined, including those of Rab11-FIP2 and Rab11-FIP3. In addition, the structures of the Rab14-FIP1 and Rab25-FIP2 complexes have been determined. All of the RBD structures contain a central parallel coiled coil in the RBD that binds to the switch 1 and switch 2 regions of the Rab. Here, the crystal structure of the uncomplexed RBD of FIP2 is presented at 2.3 Å resolution. The structure reveals antiparallel α-helices that associate through polar interactions. These include a remarkable stack of arginine residues within a four-helix bundle in the crystal lattice.

PubMed: 32744247
DOI: 10.1107/S2053230X20009164

実験手法

X-RAY DIFFRACTION (2.29 Å)