6S8C

Post-fusion conformation of the envelope protein of tick-borne encephalitis virus with longer stem

6S8C の概要

• エントリーDOI: 10.2210/pdb6s8c/pdb
• 分子名称: Genome polyprotein,Genome polyprotein (2 entities in total)
• 機能のキーワード: envelope protein, membrane fusion, stem, virus/viral protein, viral protein
• 由来する生物種: Tick-borne encephalitis virus (WESTERN SUBTYPE); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 146817.74

構造登録者

Vaney, M.C.,Rouvinski, A.,Rey, F.A. (登録日: 2019-07-09, 公開日: 2020-05-20, 最終更新日: 2024-10-16)

主引用文献

Medits, I.,Vaney, M.C.,Rouvinski, A.,Rey, M.,Chamot-Rooke, J.,Rey, F.A.,Heinz, F.X.,Stiasny, K.
Extensive flavivirus E trimer breathing accompanies stem zippering of the post-fusion hairpin.
Embo Rep., 21:e50069-e50069, 2020

PubMed Abstract: Flaviviruses enter cells by fusion with endosomal membranes through a rearrangement of the envelope protein E, a class II membrane fusion protein, into fusogenic trimers. The rod-like E subunits bend into "hairpins" to bring the fusion loops next to the C-terminal transmembrane (TM) anchors, with the TM-proximal "stem" element zippering the E trimer to force apposition of the membranes. The structure of the complete class II trimeric hairpin is known for phleboviruses but not for flaviviruses, for which the stem is only partially resolved. Here, we performed comparative analyses of E-protein trimers from the tick-borne encephalitis flavivirus with sequential stem truncations. Our thermostability and antibody-binding data suggest that the stem "zipper" ends at a characteristic flavivirus conserved sequence (CS) that cloaks the fusion loops, with the downstream segment not contributing to trimer stability. We further identified a highly dynamic behavior of E trimers C-terminally truncated upstream the CS, which, unlike fully stem-zippered trimers, undergo rapid deuterium exchange at the trimer interface. These results thus identify important "breathing" intermediates in the E-protein-driven membrane fusion process.

PubMed: 32484292
DOI: 10.15252/embr.202050069

実験手法

X-RAY DIFFRACTION (2.57 Å)