6S6V

Resting state of the E. coli Mre11-Rad50 (SbcCD) head complex bound to ATPgS

6S6V の概要

• エントリーDOI: 10.2210/pdb6s6v/pdb
• EMDBエントリー: 10107
• 分子名称: Nuclease SbcCD subunit D, Nuclease SbcCD subunit C, MANGANESE (II) ION, ... (5 entities in total)
• 機能のキーワード: nuclease, dna repair, abc-type atpase, dna double-strand breaks, dna binding protein
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 330318.47

構造登録者

Kaeshammer, L.,Saathoff, J.H.,Gut, F.,Bartho, J.,Alt, A.,Kessler, B.,Lammens, K.,Hopfner, K.P. (登録日: 2019-07-03, 公開日: 2019-09-04, 最終更新日: 2025-10-01)

主引用文献

Kashammer, L.,Saathoff, J.H.,Lammens, K.,Gut, F.,Bartho, J.,Alt, A.,Kessler, B.,Hopfner, K.P.
Mechanism of DNA End Sensing and Processing by the Mre11-Rad50 Complex.
Mol.Cell, 76:382-, 2019

PubMed Abstract: DNA double-strand breaks (DSBs) threaten genome stability throughout life and are linked to tumorigenesis in humans. To initiate DSB repair by end joining or homologous recombination, the Mre11-nuclease Rad50-ATPase complex detects and processes diverse and obstructed DNA ends, but a structural mechanism is still lacking. Here we report cryo-EM structures of the E. coli Mre11-Rad50 homolog SbcCD in resting and DNA-bound cutting states. In the resting state, Mre11's nuclease is blocked by ATP-Rad50, and the Rad50 coiled coils appear flexible. Upon DNA binding, the two coiled coils zip up into a rod and, together with the Rad50 nucleotide-binding domains, form a clamp around dsDNA. Mre11 moves to the side of Rad50, binds the DNA end, and assembles a DNA cutting channel for the nuclease reactions. The structures reveal how Mre11-Rad50 can detect and process diverse DNA ends and uncover a clamping and gating function for the coiled coils.

PubMed: 31492634
DOI: 10.1016/j.molcel.2019.07.035

実験手法

ELECTRON MICROSCOPY (3.5 Å)