6S45

Room temperature structure of the dark state of the LOV2 domain of phototropin-2 from Arabidopsis thaliana determined with a serial crystallography approach

6S45 の概要

• エントリーDOI: 10.2210/pdb6s45/pdb
• 分子名称: Phototropin-2, FLAVIN MONONUCLEOTIDE (3 entities in total)
• 機能のキーワード: lov domain, photoactive protein, flavoprotein, time-resolved crystallography, plant protein
• 由来する生物種: Arabidopsis thaliana (thale cress)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15464.25

構造登録者

Aumonier, S.,Santoni, G.,Gotthard, G.,von Stetten, D.,Leonard, G.,Royant, A. (登録日: 2019-06-26, 公開日: 2020-07-08, 最終更新日: 2024-01-24)

主引用文献

Aumonier, S.,Santoni, G.,Gotthard, G.,von Stetten, D.,Leonard, G.A.,Royant, A.
Millisecond time-resolved serial oscillation crystallography of a blue-light photoreceptor at a synchrotron.
Iucrj, 7:728-736, 2020

PubMed Abstract: The recent development of serial crystallography has popularized time-resolved crystallography as a technique to determine the structure of protein-reaction intermediate states. However, most approaches rely on the availability of thousands to millions of microcrystals. A method is reported here, using monochromatic synchrotron radiation, for the room-temperature collection, processing and merging of X-ray oscillation diffraction data from <100 samples in order to observe the build up of a photoreaction intermediate species. Using this method, we monitored with a time resolution of 63 ms how the population of a blue-light photoreceptor domain in a crystal progressively photoconverts from the dark to the light state. The series of resulting snapshots allows us to visualize in detail the gradual rearrangement of both the protein and chromophore during this process.

PubMed: 32695419
DOI: 10.1107/S2052252520007411

実験手法

X-RAY DIFFRACTION (2.2 Å)