6S1R

Structure of fission yeast Mis16 bound to histone H4

Summary for 6S1R

• Entry DOI: 10.2210/pdb6s1r/pdb
• Descriptor: Histone acetyltransferase type B subunit 2, Histone H4 (3 entities in total)
• Functional Keywords: centromere mitosis wd40 histone chaperone, cell cycle
• Biological source: Schizosaccharomyces pombe (Fission yeast); More
• Total number of polymer chains: 2
• Total formula weight: 51883.58

Authors

Lefevre, S.,Korntner-Vetter, M.,Singleton, M.R. (deposition date: 2019-06-19, release date: 2019-08-07, Last modification date: 2024-01-24)

Primary citation

Korntner-Vetter, M.,Lefevre, S.,Hu, X.W.,George, R.,Singleton, M.R.
Subunit interactions and arrangements in the fission yeast Mis16-Mis18-Mis19 complex.
Life Sci Alliance, 2:-, 2019

PubMed Abstract: Centromeric chromatin in fission yeast is distinguished by the presence of nucleosomes containing the histone H3 variant Cnp1 Cell cycle-specific deposition of Cnp1 requires the Mis16-Mis18-Mis19 complex, which is thought to direct recruitment of Scm3-chaperoned Cnp1/histone H4 dimers to DNA. Here, we present the structure of the essential Mis18 partner protein Mis19 and describe its interaction with Mis16, revealing a bipartite-binding site. We provide data on the stoichiometry and overall architecture of the complex and provide detailed insights into the Mis18-Mis19 interface.

PubMed: 31371524
DOI: 10.26508/lsa.201900408

Experimental method

X-RAY DIFFRACTION (1.8 Å)