6S10

NMR solution structure of a ProQ homolog from Legionella pneumophila

6S10 の概要

• エントリーDOI: 10.2210/pdb6s10/pdb
• NMR情報: BMRB: 27453
• 分子名称: RNA chaperone ProQ (1 entity in total)
• 機能のキーワード: proq, rna binding protein, srna
• 由来する生物種: Legionella pneumophila
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14170.93

構造登録者

Immer, C.,Hacker, C.,Woehnert, J. (登録日: 2019-06-18, 公開日: 2020-07-08, 最終更新日: 2024-06-19)

主引用文献

Immer, C.,Hacker, C.,Wohnert, J.
Solution structure and RNA-binding of a minimal ProQ-homolog from Legionella pneumophila (Lpp1663).
Rna, 26:2031-2043, 2020

PubMed Abstract: Small regulatory RNAs (sRNAs) play an important role for posttranscriptional gene regulation in bacteria. sRNAs recognize their target messenger RNAs (mRNAs) by base-pairing, which is often facilitated by interactions with the bacterial RNA-binding proteins Hfq or ProQ. The FinO/ProQ RNA-binding protein domain was first discovered in the bacterial repressor of conjugation, FinO. Since then, the functional role of FinO/ProQ-like proteins in posttranscriptional gene regulation was extensively studied in particular in the enterobacteria and and a wide range of sRNA-targets was identified for these proteins. In addition, enterobacterial ProQ homologs also recognize and protect the 3'-ends of a number of mRNAs from exonucleolytic degradation. However, the RNA-binding properties of FinO/ProQ proteins with regard to the recognition of different RNA targets are not yet fully understood. Here, we present the solution NMR structure of the so far functionally uncharacterized ProQ homolog Lpp1663 from as a newly confirmed member and a minimal model system of the FinO/ProQ protein family. In addition, we characterize the RNA-binding preferences of Lpp1663 with high resolution NMR spectroscopy and isothermal titration calorimetry (ITC). Our results suggest a binding preference for single-stranded uridine-rich RNAs in the vicinity of stable stem-loop structures. According to chemical shift perturbation experiments, the single-stranded U-rich RNAs interact mainly with a conserved RNA-binding surface on the concave site of Lpp1663.

PubMed: 32989045
DOI: 10.1261/rna.077354.120

実験手法

SOLUTION NMR