6RY3

Structure of the WUS homeodomain

6RY3 の概要

• エントリーDOI: 10.2210/pdb6ry3/pdb
• 分子名称: Protein WUSCHEL, SULFATE ION, ACETYL GROUP, ... (4 entities in total)
• 機能のキーワード: homeodomain transcription factor, dna binding protein
• 由来する生物種: Arabidopsis thaliana (thale cress)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9177.41

構造登録者

Sloan, J.J.,Wild, K.,Sinning, I. (登録日: 2019-06-10, 公開日: 2020-04-29, 最終更新日: 2024-01-24)

主引用文献

Sloan, J.,Hakenjos, J.P.,Gebert, M.,Ermakova, O.,Gumiero, A.,Stier, G.,Wild, K.,Sinning, I.,Lohmann, J.U.
Structural basis for the complex DNA binding behavior of the plant stem cell regulator WUSCHEL.
Nat Commun, 11:2223-2223, 2020

PubMed Abstract: Stem cells are one of the foundational evolutionary novelties that allowed the independent emergence of multicellularity in the plant and animal lineages. In plants, the homeodomain (HD) transcription factor WUSCHEL (WUS) is essential for the maintenance of stem cells in the shoot apical meristem. WUS has been reported to bind to diverse DNA motifs and to act as transcriptional activator and repressor. However, the mechanisms underlying this remarkable behavior have remained unclear. Here, we quantitatively delineate WUS binding to three divergent DNA motifs and resolve the relevant structural underpinnings. We show that WUS exhibits a strong binding preference for TGAA repeat sequences, while retaining the ability to weakly bind to TAAT elements. This behavior is attributable to the formation of dimers through interactions of specific residues in the HD that stabilize WUS DNA interaction. Our results provide a mechanistic basis for dissecting WUS dependent regulatory networks in plant stem cell control.

PubMed: 32376862
DOI: 10.1038/s41467-020-16024-y

実験手法

X-RAY DIFFRACTION (1.374 Å)