6RXT

Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaetomium thermophilum, state A

これはPDB形式変換不可エントリーです。

6RXT の概要

• エントリーDOI: 10.2210/pdb6rxt/pdb
• EMDBエントリー: 10051
• 分子名称: Periodic tryptophan protein 2-like protein, Utp13", Utp14, ... (55 entities in total)
• 機能のキーワード: ribosome, ribosome biogenesis, rrna
• 由来する生物種: Chaetomium thermophilum; 詳細
• タンパク質・核酸の鎖数: 57
• 化学式量合計: 3905713.99

構造登録者

Cheng, J.,Kellner, N.,Griesel, S.,Berninghausen, O.,Beckmann, R.,Hurt, E. (登録日: 2019-06-10, 公開日: 2019-08-14, 最終更新日: 2024-05-22)

主引用文献

Cheng, J.,Bassler, J.,Fischer, P.,Lau, B.,Kellner, N.,Kunze, R.,Griesel, S.,Kallas, M.,Berninghausen, O.,Strauss, D.,Beckmann, R.,Hurt, E.
Thermophile 90S Pre-ribosome Structures Reveal the Reverse Order of Co-transcriptional 18S rRNA Subdomain Integration.
Mol.Cell, 75:1256-1269.e7, 2019

PubMed Abstract: Eukaryotic ribosome biogenesis involves RNA folding and processing that depend on assembly factors and small nucleolar RNAs (snoRNAs). The 90S (SSU-processome) is the earliest pre-ribosome structurally analyzed, which was suggested to assemble stepwise along the growing pre-rRNA from 5' > 3', but this directionality may not be accurate. Here, by analyzing the structure of a series of 90S assembly intermediates from Chaetomium thermophilum, we discover a reverse order of 18S rRNA subdomain incorporation. Large parts of the 18S rRNA 3' and central domains assemble first into the 90S before the 5' domain is integrated. This final incorporation depends on a contact between a heterotrimer Enp2-Bfr2-Lcp5 recruited to the flexible 5' domain and Kre33, which reconstitutes the Kre33-Enp-Brf2-Lcp5 module on the compacted 90S. Keeping the 5' domain temporarily segregated from the 90S scaffold could provide extra time to complete the multifaceted 5' domain folding, which depends on a distinct set of snoRNAs and processing factors.

PubMed: 31378463
DOI: 10.1016/j.molcel.2019.06.032

実験手法

ELECTRON MICROSCOPY (7 Å)