6RXQ

Crystal structure of CobB Ac2 (A76G,I131C,V162A) in complex with H4K16Cr-2'OH-ADPr peptide intermediate after soaking

Summary for 6RXQ

• Entry DOI: 10.2210/pdb6rxq/pdb
• Descriptor: NAD-dependent protein deacylase, Histone H4, [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{S})-4-[(~{E})-but-2-enoxy]-3,5-bis(oxidanyl)oxolan-2-yl]methyl hydrogen phosphate, ... (4 entities in total)
• Functional Keywords: deacylase, nad-dependent, nad, intermediate, crotonyl, lysine, hydrolase
• Biological source: Escherichia coli (strain K12); More
• Total number of polymer chains: 8
• Total formula weight: 119750.59

Authors

Spinck, M.,Gasper, R.,Neumann, H. (deposition date: 2019-06-08, release date: 2020-04-15, Last modification date: 2024-10-09)

Primary citation

Spinck, M.,Neumann-Staubitz, P.,Ecke, M.,Gasper, R.,Neumann, H.
Evolved, Selective Erasers of Distinct Lysine Acylations.
Angew.Chem.Int.Ed.Engl., 59:11142-11149, 2020

PubMed Abstract: Lysine acylations, a family of diverse protein modifications varying in acyl-group length, charge, and saturation, are linked to many important physiological processes. Only a small set of substrate-promiscuous lysine acetyltransferases and deacetylases (KDACs) install and remove this vast variety of modifications. Engineered KDACs that remove only one type of acylation would help to dissect the different contributions of distinct acylations. We developed a bacterial selection system for the directed evolution of KDACs and identified variants up to 400 times more selective for butyryl-lysine compared to crotonyl-lysine. Structural analyses revealed that the enzyme adopts different conformational states depending on the type of acylation of the bound peptide. We used the butyryl-selective KDAC variant to shift the cellular acylation spectrum towards increased lysine crotonylation. These new enzymes will help in dissecting the roles of different lysine acylations in cell physiology.

PubMed: 32187803
DOI: 10.1002/anie.202002899

Experimental method

X-RAY DIFFRACTION (1.7 Å)