6RWG
Structure of HIV-1 CAcSP1NC mutant(W41A,M42A) interacting with maturation inhibitor EP39
Summary for 6RWG
| Entry DOI | 10.2210/pdb6rwg/pdb |
| NMR Information | BMRB: 34409 |
| Descriptor | Gag polyprotein, ZINC ION (2 entities in total) |
| Functional Keywords | gag, hiv-1, maturation inhibitor, ep39, structural genomics, psi-biology, structural genomics consortium, sgc, viral protein |
| Biological source | Human immunodeficiency virus 1 |
| Total number of polymer chains | 1 |
| Total formula weight | 17576.96 |
| Authors | Chen, X.,Coric, P.,Larue, V.,Nonin-Lecomte, S.,Bouaziz, S.,Structural Genomics Consortium (SGC) (deposition date: 2019-06-05, release date: 2020-05-20, Last modification date: 2024-06-19) |
| Primary citation | Chen, X.,Coric, P.,Larue, V.,Turcaud, S.,Wang, X.,Nonin-Lecomte, S.,Bouaziz, S. The HIV-1 maturation inhibitor, EP39, interferes with the dynamic helix-coil equilibrium of the CA-SP1 junction of Gag. Eur.J.Med.Chem., 204:112634-112634, 2020 Cited by PubMed Abstract: During the maturation of HIV-1 particle, the Gag polyprotein is cleaved into several proteins by the HIV-1 protease. These proteins rearrange to form infectious virus particles. In this study, the solution structure and dynamics of a monomeric mutated domain encompassing the C-terminal of capsid, the spacer peptide SP1 and the nucleocapsid from Gag was characterized by Nuclear Magnetic Resonance in the presence of maturation inhibitor EP39, a more hydro-soluble derivative of BVM. We show that the binding of EP39 decreases the dynamics of CA-SP1 junction, especially the QVT motif in SP1, and perturbs the natural coil-helix equilibrium on both sides of the SP1 domain by stabilizing the transient alpha helical structure. Our results provide new insight into the structure and dynamics of the SP1 domain and how HIV-1 maturation inhibitors interfere with this domain. They offer additional clues for the development of new second generation inhibitors targeting HIV-1 maturation. PubMed: 32717487DOI: 10.1016/j.ejmech.2020.112634 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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