6RWG

Structure of HIV-1 CAcSP1NC mutant(W41A,M42A) interacting with maturation inhibitor EP39

6RWG の概要

• エントリーDOI: 10.2210/pdb6rwg/pdb
• NMR情報: BMRB: 34409
• 分子名称: Gag polyprotein, ZINC ION (2 entities in total)
• 機能のキーワード: gag, hiv-1, maturation inhibitor, ep39, structural genomics, psi-biology, structural genomics consortium, sgc, viral protein
• 由来する生物種: Human immunodeficiency virus 1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17576.96

構造登録者

Chen, X.,Coric, P.,Larue, V.,Nonin-Lecomte, S.,Bouaziz, S.,Structural Genomics Consortium (SGC) (登録日: 2019-06-05, 公開日: 2020-05-20, 最終更新日: 2024-06-19)

主引用文献

Chen, X.,Coric, P.,Larue, V.,Turcaud, S.,Wang, X.,Nonin-Lecomte, S.,Bouaziz, S.
The HIV-1 maturation inhibitor, EP39, interferes with the dynamic helix-coil equilibrium of the CA-SP1 junction of Gag.
Eur.J.Med.Chem., 204:112634-112634, 2020

PubMed Abstract: During the maturation of HIV-1 particle, the Gag polyprotein is cleaved into several proteins by the HIV-1 protease. These proteins rearrange to form infectious virus particles. In this study, the solution structure and dynamics of a monomeric mutated domain encompassing the C-terminal of capsid, the spacer peptide SP1 and the nucleocapsid from Gag was characterized by Nuclear Magnetic Resonance in the presence of maturation inhibitor EP39, a more hydro-soluble derivative of BVM. We show that the binding of EP39 decreases the dynamics of CA-SP1 junction, especially the QVT motif in SP1, and perturbs the natural coil-helix equilibrium on both sides of the SP1 domain by stabilizing the transient alpha helical structure. Our results provide new insight into the structure and dynamics of the SP1 domain and how HIV-1 maturation inhibitors interfere with this domain. They offer additional clues for the development of new second generation inhibitors targeting HIV-1 maturation.

PubMed: 32717487
DOI: 10.1016/j.ejmech.2020.112634

実験手法

SOLUTION NMR