6RVA

STRUCTURE OF [ASP58]-IGF-I ANALOGUE

6RVA の概要

• エントリーDOI: 10.2210/pdb6rva/pdb
• NMR情報: BMRB: 34408
• 分子名称: Insulin-like growth factor I (1 entity in total)
• 機能のキーワード: igf-i analogue, hormone
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7706.78

構造登録者

Jiracek, J.,Zakova, L.,Socha, O. (登録日: 2019-05-31, 公開日: 2019-10-02, 最終更新日: 2023-06-14)

主引用文献

Machackova, K.,Mlcochova, K.,Potalitsyn, P.,Hankova, K.,Socha, O.,Budesinsky, M.,Muzdalo, A.,Lepsik, M.,Cernekova, M.,Radosavljevic, J.,Fabry, M.,Mitrova, K.,Chrudinova, M.,Lin, J.,Yurenko, Y.,Hobza, P.,Selicharova, I.,Zakova, L.,Jiracek, J.
Mutations at hypothetical binding site 2 in insulin and insulin-like growth factors 1 and 2 result in receptor- and hormone-specific responses.
J.Biol.Chem., 294:17371-17382, 2019

PubMed Abstract: Information on how insulin and insulin-like growth factors 1 and 2 (IGF-1 and -2) activate insulin receptors (IR-A and -B) and the IGF-1 receptor (IGF-1R) is crucial for understanding the difference in the biological activities of these peptide hormones. Cryo-EM studies have revealed that insulin uses its binding sites 1 and 2 to interact with IR-A and have identified several critical residues in binding site 2. However, mutagenesis studies suggest that Ile-A10, Ser-A12, Leu-A13, and Glu-A17 also belong to insulin's site 2. Here, to resolve this discrepancy, we mutated these insulin residues and the equivalent residues in IGFs. Our findings revealed that equivalent mutations in the hormones can result in differential biological effects and that these effects can be receptor-specific. We noted that the insulin positions A10 and A17 are important for its binding to IR-A and IR-B and IGF-1R and that A13 is important only for IR-A and IR-B binding. The IGF-1/IGF-2 positions 51/50 and 54/53 did not appear to play critical roles in receptor binding, but mutations at IGF-1 position 58 and IGF-2 position 57 affected the binding. We propose that IGF-1 Glu-58 interacts with IGF-1R Arg-704 and belongs to IGF-1 site 1, a finding supported by the NMR structure of the less active Asp-58-IGF-1 variant. Computational analyses indicated that the aforementioned mutations can affect internal insulin dynamics and inhibit adoption of a receptor-bound conformation, important for binding to receptor site 1. We provide a molecular model and alternative hypotheses for how the mutated insulin residues affect activity.

PubMed: 31558604
DOI: 10.1074/jbc.RA119.010072

実験手法

SOLUTION NMR