6RV5

X-ray structure of the levansucrase from Erwinia tasmaniensis in complex with levanbiose

6RV5 の概要

• エントリーDOI: 10.2210/pdb6rv5/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900051
• 分子名称: Levansucrase (Beta-D-fructofuranosyl transferase), beta-D-fructofuranose-(2-6)-beta-D-fructofuranose, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: levansucrase, levanbiose, fructosyltransferase, fructans production., transferase
• 由来する生物種: Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 47625.38

構造登録者

Polsinelli, I.,Caliandro, R.,Demitri, N.,Benini, S. (登録日: 2019-05-31, 公開日: 2020-04-01, 最終更新日: 2024-01-31)

主引用文献

Polsinelli, I.,Caliandro, R.,Demitri, N.,Benini, S.
The Structure of Sucrose-Soaked Levansucrase Crystals fromErwinia tasmaniensisreveals a Binding Pocket for Levanbiose.
Int J Mol Sci, 21:-, 2019

PubMed Abstract: Given its potential role in the synthesis of novel prebiotics and applications in the pharmaceutical industry, a strong interest has developed in the enzyme levansucrase (LSC, EC 2.4.1.10). LSC catalyzes both the hydrolysis of sucrose (or sucroselike substrates) and the transfructosylation of a wide range of acceptors. LSC from the Gram-negative bacterium (EtLSC) is an interesting biocatalyst due to its high-yield production of fructooligosaccharides (FOSs). In order to learn more about the process of chain elongation, we obtained the crystal structure of EtLSC in complex with levanbiose (LBS). LBS is an FOS intermediate formed during the synthesis of longer-chain FOSs and levan. Analysis of the LBS binding pocket revealed that its structure was conserved in several related species. The binding pocket discovered in this crystal structure is an ideal target for future mutagenesis studies in order to understand its biological relevance and to engineer LSCs into tailored products.

PubMed: 31877648
DOI: 10.3390/ijms21010083

実験手法

X-RAY DIFFRACTION (1.58 Å)