6RUM

Crystal structure of GFP-LAMA-G97 - a GFP enhancer nanobody with cpDHFR insertion and TMP and NADPH

6RUM の概要

• エントリーDOI: 10.2210/pdb6rum/pdb
• 関連するPDBエントリー: 6RUL
• 分子名称: GFP-LAMA-G97 a GFP enhancer nanobody with cpDHFR insertion, TRIMETHOPRIM, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, ... (7 entities in total)
• 機能のキーワード: lama, nanobody, circular permutant of dhfr, tmp, nadph, protein binding
• 由来する生物種: lama glama; 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32427.09

構造登録者

Farrants, H.,Tarnawski, M.,Mueller, T.G.,Otsuka, S.,Hiblot, J.,Koch, B.,Kueblbeck, M.,Kraeusslich, H.-G.,Ellenberg, J.,Johnsson, K. (登録日: 2019-05-28, 公開日: 2020-02-12, 最終更新日: 2024-01-24)

主引用文献

Farrants, H.,Tarnawski, M.,Muller, T.G.,Otsuka, S.,Hiblot, J.,Koch, B.,Kueblbeck, M.,Krausslich, H.G.,Ellenberg, J.,Johnsson, K.
Chemogenetic Control of Nanobodies.
Nat.Methods, 17:279-282, 2020

PubMed Abstract: We introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switched on and off with small molecules. By controlling the cellular localization of GFP fusion proteins, the engineered nanobody allows interrogation of their roles in basic biological processes, an approach that should be applicable to numerous previously described GFP fusions. We also outline how the binding affinities of other nanobodies can be controlled by small molecules.

PubMed: 32066961
DOI: 10.1038/s41592-020-0746-7

実験手法

X-RAY DIFFRACTION (1.6 Å)