6RSQ

Helical folded domain of mouse CAP1

6RSQ の概要

• エントリーDOI: 10.2210/pdb6rsq/pdb
• 分子名称: Adenylyl cyclase-associated protein 1, GLYCEROL (3 entities in total)
• 機能のキーワード: actin binding protein, contractile protein
• 由来する生物種: Mus musculus (house mouse)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 90022.86

構造登録者

Kotila, T.,Kogan, K.,Lappalainen, P. (登録日: 2019-05-22, 公開日: 2019-11-27, 最終更新日: 2024-11-06)

主引用文献

Kotila, T.,Wioland, H.,Enkavi, G.,Kogan, K.,Vattulainen, I.,Jegou, A.,Romet-Lemonne, G.,Lappalainen, P.
Mechanism of synergistic actin filament pointed end depolymerization by cyclase-associated protein and cofilin.
Nat Commun, 10:5320-5320, 2019

PubMed Abstract: The ability of cells to generate forces through actin filament turnover was an early adaptation in evolution. While much is known about how actin filaments grow, mechanisms of their disassembly are incompletely understood. The best-characterized actin disassembly factors are the cofilin family proteins, which increase cytoskeletal dynamics by severing actin filaments. However, the mechanism by which severed actin filaments are recycled back to monomeric form has remained enigmatic. We report that cyclase-associated-protein (CAP) works in synergy with cofilin to accelerate actin filament depolymerization by nearly 100-fold. Structural work uncovers the molecular mechanism by which CAP interacts with actin filament pointed end to destabilize the interface between terminal actin subunits, and subsequently recycles the newly-depolymerized actin monomer for the next round of filament assembly. These findings establish CAP as a molecular machine promoting rapid actin filament depolymerization and monomer recycling, and explain why CAP is critical for actin-dependent processes in all eukaryotes.

PubMed: 31757941
DOI: 10.1038/s41467-019-13213-2

実験手法

X-RAY DIFFRACTION (2.37 Å)