6RRD

RNA Polymerase I Pre-initiation complex DNA opening intermediate 1

6RRD の概要

• エントリーDOI: 10.2210/pdb6rrd/pdb
• EMDBエントリー: 4987
• 分子名称: Template strand, DNA-directed RNA polymerase I subunit RPA135, DNA-directed RNA polymerases I and III subunit RPAC1, ... (21 entities in total)
• 機能のキーワード: rna polymerase i, pre-initiation complex, pic, open complex, oc, core factor, cf, rrn3, transcription, dna opening
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 20
• 化学式量合計: 927893.57

構造登録者

Mueller, C.W.,Sadian, Y.,Tafur, L. (登録日: 2019-05-17, 公開日: 2019-12-11, 最終更新日: 2024-05-22)

主引用文献

Sadian, Y.,Baudin, F.,Tafur, L.,Murciano, B.,Wetzel, R.,Weis, F.,Muller, C.W.
Molecular insight into RNA polymerase I promoter recognition and promoter melting.
Nat Commun, 10:5543-5543, 2019

PubMed Abstract: RNA polymerase I (Pol I) assembles with core factor (CF) and Rrn3 on the rDNA core promoter for transcription initiation. Here, we report cryo-EM structures of closed, intermediate and open Pol I initiation complexes from 2.7 to 3.7 Å resolution to visualize Pol I promoter melting and to structurally and biochemically characterize the recognition mechanism of Pol I promoter DNA. In the closed complex, double-stranded DNA runs outside the DNA-binding cleft. Rotation of CF and upstream DNA with respect to Pol I and Rrn3 results in the spontaneous loading and opening of the promoter followed by cleft closure and positioning of the Pol I A49 tandem winged helix domain (tWH) onto DNA. Conformational rearrangement of A49 tWH leads to a clash with Rrn3 to initiate complex disassembly and promoter escape. Comprehensive insight into the Pol I transcription initiation cycle allows comparisons with promoter opening by Pol II and Pol III.

PubMed: 31804486
DOI: 10.1038/s41467-019-13510-w

実験手法

ELECTRON MICROSCOPY (3.1 Å)