6RNB

Liquid Application Method for time-resolved Analyses (LAMA) by serial synchrotron crystallography, Lysozyme with GlcNAc3 50ms diffusion time

6RNB の概要

• エントリーDOI: 10.2210/pdb6rnb/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900017
• 分子名称: Lysozyme C, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: lysozyme, complex, diffusion, ligand, hydrolase
• 由来する生物種: Gallus gallus (Chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15052.65

構造登録者

Mehrabi, P.,Schulz, E.C.,Miller, R.J.D. (登録日: 2019-05-08, 公開日: 2019-10-02, 最終更新日: 2024-11-20)

主引用文献

Mehrabi, P.,Schulz, E.C.,Agthe, M.,Horrell, S.,Bourenkov, G.,von Stetten, D.,Leimkohl, J.P.,Schikora, H.,Schneider, T.R.,Pearson, A.R.,Tellkamp, F.,Miller, R.J.D.
Liquid application method for time-resolved analyses by serial synchrotron crystallography.
Nat.Methods, 16:979-982, 2019

PubMed Abstract: We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase.

PubMed: 31527838
DOI: 10.1038/s41592-019-0553-1

実験手法

X-RAY DIFFRACTION (1.699 Å)