6RM3

Evolutionary compaction and adaptation visualized by the structure of the dormant microsporidian ribosome

これはPDB形式変換不可エントリーです。

6RM3 の概要

• エントリーDOI: 10.2210/pdb6rm3/pdb
• EMDBエントリー: 4931 4932 4933 4934 4935
• 分子名称: 16S rRNA, eS27, uL4, ... (80 entities in total)
• 機能のキーワード: microsporidia, ribosome, intracellular parasite
• 由来する生物種: Vairimorpha necatrix; 詳細
• タンパク質・核酸の鎖数: 78
• 化学式量合計: 2572745.53

構造登録者

Barandun, J.,Hunziker, M.,Vossbrinck, C.R.,Klinge, S. (登録日: 2019-05-05, 公開日: 2019-07-10, 最終更新日: 2024-07-10)

主引用文献

Barandun, J.,Hunziker, M.,Vossbrinck, C.R.,Klinge, S.
Evolutionary compaction and adaptation visualized by the structure of the dormant microsporidian ribosome.
Nat Microbiol, 4:1798-1804, 2019

PubMed Abstract: Microsporidia are eukaryotic parasites that infect essentially all animal species, including many of agricultural importance, and are significant opportunistic parasites of humans. They are characterized by having a specialized infection apparatus, an obligate intracellular lifestyle, rudimentary mitochondria and the smallest known eukaryotic genomes. Extreme genome compaction led to minimal gene sizes affecting even conserved ancient complexes such as the ribosome. In the present study, the cryo-electron microscopy structure of the ribosome from the microsporidium Vairimorpha necatrix is presented, which illustrates how genome compaction has resulted in the smallest known eukaryotic cytoplasmic ribosome. Selection pressure led to the loss of two ribosomal proteins and removal of essentially all eukaryote-specific ribosomal RNA (rRNA) expansion segments, reducing the rRNA to a functionally conserved core. The structure highlights how one microsporidia-specific and several repurposed existing ribosomal proteins compensate for the extensive rRNA reduction. The microsporidian ribosome is kept in an inactive state by two previously uncharacterized dormancy factors that specifically target the functionally important E-site, P-site and polypeptide exit tunnel. The present study illustrates the distinct effects of evolutionary pressure on RNA and protein-coding genes, provides a mechanism for ribosome inhibition and can serve as a structural basis for the development of inhibitors against microsporidian parasites.

PubMed: 31332387
DOI: 10.1038/s41564-019-0514-6

実験手法

ELECTRON MICROSCOPY (3.4 Å)