6RK5

Inter-dimeric interface controls function and stability of S-methionine adenosyltransferase from U. urealiticum

Summary for 6RK5

• Entry DOI: 10.2210/pdb6rk5/pdb
• Related: 6RJS
• Descriptor: Methionine adenosyltransferase (2 entities in total)
• Functional Keywords: synthetase, transferase
• Biological source: Ureaplasma urealyticum serovar 7 str. ATCC 27819
• Total number of polymer chains: 4
• Total formula weight: 171522.41

Authors

Shahar, A.,Zarivach, R.,Bershtein, S.,Kleiner, D.,Shmulevich, F. (deposition date: 2019-04-30, release date: 2019-09-25, Last modification date: 2024-01-24)

Primary citation

Kleiner, D.,Shmulevich, F.,Zarivach, R.,Shahar, A.,Sharon, M.,Ben-Nissan, G.,Bershtein, S.
The interdimeric interface controls function and stability of Ureaplasma urealiticum methionine S-adenosyltransferase.
J.Mol.Biol., 431:4796-4816, 2019

PubMed Abstract: Methionine S-adenosyltransferases (MATs) are predominantly homotetramers, comprised of dimers of dimers. The larger, highly conserved intradimeric interface harbors two active sites, making the dimer the obligatory functional unit. However, functionality of the smaller, more diverged, and recently evolved interdimeric interface is largely unknown. Here, we show that the interdimeric interface of Ureaplasmaurealiticum MAT has evolved to control the catalytic activity and structural integrity of the homotetramer in response to product accumulation. When all four active sites are occupied with the product, S-adenosylmethionine (SAM), binding of four additional SAM molecules to the interdimeric interface prompts a ∼45° shift in the dimer orientation and a concomitant ∼60% increase in the interface area. This rearrangement inhibits the enzymatic activity by locking the flexible active site loops in a closed state and renders the tetramer resistant to proteolytic degradation. Our findings suggest that the interdimeric interface of MATs is subject to rapid evolutionary changes that tailor the molecular properties of the entire homotetramer to the specific needs of the organism.

PubMed: 31520601
DOI: 10.1016/j.jmb.2019.09.003

Experimental method

X-RAY DIFFRACTION (2.6 Å)