6RJH

3D structure of horse spleen apoferritin determined using multifunctional graphene supports for electron cryomicroscopy

Summary for 6RJH

• Entry DOI: 10.2210/pdb6rjh/pdb
• EMDB information: 4905
• Descriptor: Ferritin light chain (1 entity in total)
• Functional Keywords: metal transport
• Biological source: Equus caballus (Horse)
• Total number of polymer chains: 24
• Total formula weight: 468770.71

Authors

Naydenova, K.,Peet, M.J.,Russo, C.J. (deposition date: 2019-04-26, release date: 2019-06-05, Last modification date: 2024-05-22)

Primary citation

Naydenova, K.,Peet, M.J.,Russo, C.J.
Multifunctional graphene supports for electron cryomicroscopy.
Proc.Natl.Acad.Sci.USA, 116:11718-11724, 2019

PubMed Abstract: With recent technological advances, the atomic resolution structure of any purified biomolecular complex can, in principle, be determined by single-particle electron cryomicroscopy (cryoEM). In practice, the primary barrier to structure determination is the preparation of a frozen specimen suitable for high-resolution imaging. To address this, we present a multifunctional specimen support for cryoEM, comprising large-crystal monolayer graphene suspended across the surface of an ultrastable gold specimen support. Using a low-energy plasma surface modification system, we tune the surface of this support to the specimen by patterning a range of covalent functionalizations across the graphene layer on a single grid. This support design reduces specimen movement during imaging, improves image quality, and allows high-resolution structure determination with a minimum of material and data.

PubMed: 31127045
DOI: 10.1073/pnas.1904766116

Experimental method

ELECTRON MICROSCOPY (2.1 Å)