6RIA

Bactofilin from Thermus thermophilus, F105R mutant crystal structure

6RIA の概要

• エントリーDOI: 10.2210/pdb6ria/pdb
• 分子名称: bactofilin (1 entity in total)
• 機能のキーワード: prokaryotic cytoskeletons, protein fibril
• 由来する生物種: Thermus thermophilus
• タンパク質・核酸の鎖数: 32
• 化学式量合計: 420546.40

構造登録者

Lowe, J.,Gonzalez Llamazares, A. (登録日: 2019-04-23, 公開日: 2019-07-17, 最終更新日: 2024-05-15)

主引用文献

Deng, X.,Gonzalez Llamazares, A.,Wagstaff, J.M.,Hale, V.L.,Cannone, G.,McLaughlin, S.H.,Kureisaite-Ciziene, D.,Lowe, J.
The structure of bactofilin filaments reveals their mode of membrane binding and lack of polarity.
Nat Microbiol, 4:2357-2368, 2019

PubMed Abstract: Bactofilins are small β-helical proteins that form cytoskeletal filaments in a range of bacteria. Bactofilins have diverse functions, from cell stalk formation in Caulobacter crescentus to chromosome segregation and motility in Myxococcus xanthus. However, the precise molecular architecture of bactofilin filaments has remained unclear. Here, sequence analysis and electron microscopy results reveal that, in addition to being widely distributed across bacteria and archaea, bactofilins are also present in a few eukaryotic lineages such as the Oomycetes. Electron cryomicroscopy analysis demonstrated that the sole bactofilin from Thermus thermophilus (TtBac) forms constitutive filaments that polymerize through end-to-end association of the β-helical domains. Using a nanobody, we determined the near-atomic filament structure, showing that the filaments are non-polar. A polymerization-impairing mutation enabled crystallization and structure determination, while reaffirming the lack of polarity and the strength of the β-stacking interface. To confirm the generality of the lack of polarity, we performed coevolutionary analysis on a large set of sequences. Finally, we determined that the widely conserved N-terminal disordered tail of TtBac is responsible for direct binding to lipid membranes, both on liposomes and in Escherichia coli cells. Membrane binding is probably a common feature of these widespread but only recently discovered filaments of the prokaryotic cytoskeleton.

PubMed: 31501539
DOI: 10.1038/s41564-019-0544-0

実験手法

X-RAY DIFFRACTION (3.5 Å)