6RGI

Partially unfolded cytochrome c in complex with sulfonatocalix[6]arene

6RGI の概要

• エントリーDOI: 10.2210/pdb6rgi/pdb
• 分子名称: Cytochrome c iso-1, HEME C, p-sulfonatocalix[6]arene, ... (5 entities in total)
• 機能のキーワード: molecular glues, calixarene, protein assembly, supramolecular chemistry, electron transport
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13840.42

構造登録者

Engilberge, S.,Rennie, M.L.,Crowley, P.B. (登録日: 2019-04-16, 公開日: 2019-07-10, 最終更新日: 2024-11-06)

主引用文献

Engilberge, S.,Rennie, M.L.,Crowley, P.B.
Calixarene capture of partially unfolded cytochrome c.
Febs Lett., 593:2112-2117, 2019

PubMed Abstract: Supramolecular receptors such as water-soluble calixarenes are in development as 'molecular glues' for protein assembly. Here, we obtained cocrystals of sulfonato-calix[6]arene (sclx ) and yeast cytochrome c (cytc) in the presence of imidazole. A crystal structure at 2.65 Å resolution reveals major structural rearrangement and disorder in imidazole-bound cytc. The largest protein-calixarene interface involves 440 Å of the protein surface with key contacts at Arg13, Lys73, and Lys79. These lysines participate in alkaline transitions of cytc and are part of Ω-loop D, which is substantially restructured in the complex with sclx . The structural modification also includes Ω-loop C, which is disordered (residues 41-55 inclusive). These results suggest the possibility of using supramolecular scaffolds to trap partially disordered proteins.

PubMed: 31254353
DOI: 10.1002/1873-3468.13512

実験手法

X-RAY DIFFRACTION (2.64 Å)