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6RCL

Crystal structure of REXO2-D199A-AA

Summary for 6RCL
Entry DOI10.2210/pdb6rcl/pdb
Related6RCI
DescriptorOligoribonuclease, mitochondrial, RNA (5'-R(P*AP*A)-3'), ZINC ION, ... (5 entities in total)
Functional Keywordsmitochondria, oligoribonuclease, rexo2, hydrolase
Biological sourceHomo sapiens (Human)
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Total number of polymer chains3
Total formula weight42405.07
Authors
Spahr, H.,Nicholls, T.J.,Larsson, N.G.,Gustafsson, C.M. (deposition date: 2019-04-11, release date: 2019-10-09, Last modification date: 2024-05-15)
Primary citationNicholls, T.J.,Spahr, H.,Jiang, S.,Siira, S.J.,Koolmeister, C.,Sharma, S.,Kauppila, J.H.K.,Jiang, M.,Kaever, V.,Rackham, O.,Chabes, A.,Falkenberg, M.,Filipovska, A.,Larsson, N.G.,Gustafsson, C.M.
Dinucleotide Degradation by REXO2 Maintains Promoter Specificity in Mammalian Mitochondria.
Mol.Cell, 76:784-796.e6, 2019
Cited by
PubMed Abstract: Oligoribonucleases are conserved enzymes that degrade short RNA molecules of up to 5 nt in length and are assumed to constitute the final stage of RNA turnover. Here we demonstrate that REXO2 is a specialized dinucleotide-degrading enzyme that shows no preference between RNA and DNA dinucleotide substrates. A heart- and skeletal-muscle-specific knockout mouse displays elevated dinucleotide levels and alterations in gene expression patterns indicative of aberrant dinucleotide-primed transcription initiation. We find that dinucleotides act as potent stimulators of mitochondrial transcription initiation in vitro. Our data demonstrate that increased levels of dinucleotides can be used to initiate transcription, leading to an increase in transcription levels from both mitochondrial promoters and other, nonspecific sequence elements in mitochondrial DNA. Efficient RNA turnover by REXO2 is thus required to maintain promoter specificity and proper regulation of transcription in mammalian mitochondria.
PubMed: 31588022
DOI: 10.1016/j.molcel.2019.09.010
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.97 Å)
Structure validation

227561

数据于2024-11-20公开中

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