6R9G

Structural basis of transcription inhibition by the DNA mimic Ocr protein of bacteriophage T7

6R9G の概要

• エントリーDOI: 10.2210/pdb6r9g/pdb
• EMDBエントリー: 4769 4770
• 分子名称: DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (5 entities in total)
• 機能のキーワード: transcription, rna polymerase, transcription inhibition, ocr, bacteriophage
• 由来する生物種: Escherichia coli (strain K12); 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 416037.28

構造登録者

Ye, F.Z.,Zhang, X.D. (登録日: 2019-04-03, 公開日: 2020-02-26, 最終更新日: 2024-05-22)

主引用文献

Ye, F.,Kotta-Loizou, I.,Jovanovic, M.,Liu, X.,Dryden, D.T.,Buck, M.,Zhang, X.
Structural basis of transcription inhibition by the DNA mimic protein Ocr of bacteriophage T7.
Elife, 9:-, 2020

PubMed Abstract: Bacteriophage T7 infects and evades the host restriction/modification system. The Ocr protein of T7 was shown to exist as a dimer mimicking DNA and to bind to host restriction enzymes, thus preventing the degradation of the viral genome by the host. Here we report that Ocr can also inhibit host transcription by directly binding to bacterial RNA polymerase (RNAP) and competing with the recruitment of RNAP by sigma factors. Using cryo electron microscopy, we determined the structures of Ocr bound to RNAP. The structures show that an Ocr dimer binds to RNAP in the cleft, where key regions of sigma bind and where DNA resides during transcription synthesis, thus providing a structural basis for the transcription inhibition. Our results reveal the versatility of Ocr in interfering with host systems and suggest possible strategies that could be exploited in adopting DNA mimicry as a basis for forming novel antibiotics.

PubMed: 32039758
DOI: 10.7554/eLife.52125

実験手法

ELECTRON MICROSCOPY (3.7 Å)