6R7G

Atomic structure of potato virus X, the prototype of the Alphaflexiviridae family

6R7G の概要

• エントリーDOI: 10.2210/pdb6r7g/pdb
• EMDBエントリー: 4740
• 分子名称: Coat protein, polyU RNA (3 entities in total)
• 機能のキーワード: virus, coat protein, rna
• 由来する生物種: Potato virus X; 詳細
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 313834.17

構造登録者

Grinzato, A.,Kandiah, E.,Lico, C.,Betti, C.,Baschieri, S.,Zanotti, G. (登録日: 2019-03-28, 公開日: 2020-03-18, 最終更新日: 2025-07-02)

主引用文献

Grinzato, A.,Kandiah, E.,Lico, C.,Betti, C.,Baschieri, S.,Zanotti, G.
Atomic structure of potato virus X, the prototype of the Alphaflexiviridae family.
Nat.Chem.Biol., 16:564-569, 2020

PubMed Abstract: Potato virus X (PVX) is a positive-sense single-stranded RNA (ssRNA) filamentous plant virus belonging to the Alphaflexiviridae family, considered in recent years as a tool for nanotechnology applications. We present the cryo-electron microscopy structure of the PVX particle at a resolution of 2.2 Å. The well-defined density of the coat proteins and of the genomic RNA allowed a detailed analysis of protein-RNA interactions, including those mediated by solvent molecules. The particle is formed by repeated segments made of 8.8 coat proteins, forming a left-handed helical structure. The RNA runs in an internal crevice along the virion, packaged in 5-nucleotide repeats in which the first four bases are stacked in the classical way, while the fifth is rotated and nearly perpendicular. The resolution of the structure described here suggests a mechanism for the virion assembly and potentially provides a platform for the rational design of antiviral compounds and for the use of PVX in nanotechnology.

PubMed: 32203412
DOI: 10.1038/s41589-020-0502-4

実験手法

ELECTRON MICROSCOPY (2.2 Å)