6R2M

Crystal structure of PssZ from Listeria monocytogenes

Summary for 6R2M

• Entry DOI: 10.2210/pdb6r2m/pdb
• Descriptor: Glycoside transferase (2 entities in total)
• Functional Keywords: glycosylhydrolase, exopolysacharide, monomer, extracellular, hydrolase
• Biological source: Listeria monocytogenes
• Total number of polymer chains: 2
• Total formula weight: 75684.35

Authors

Wu, H.,Cheng, J.,Qiao, S.,Li, D.,Ma, L. (deposition date: 2019-03-18, release date: 2019-07-24, Last modification date: 2024-05-15)

Primary citation

Wu, H.,Qiao, S.,Li, D.,Guo, L.,Zhu, M.,Ma, L.Z.
Crystal structure of the glycoside hydrolase PssZ from Listeria monocytogenes.
Acta Crystallogr.,Sect.F, 75:501-506, 2019

PubMed Abstract: Biofilms are microbial communities that are embedded in the extracellular matrix. The exopolysaccharide (EPS) is a key component of the biofilm matrix that maintains the structure of the biofilm and protects the bacteria from antimicrobials. Microbial glycoside hydrolases have been exploited to disrupt biofilms by breaking down EPSs. PssZ has recently been identified as a glycoside hydrolase that can disperse aggregates of Listeria monocytogenes. In this study, the crystal structure of PssZ has been determined at 1.6 Å resolution. PssZ belongs to glycoside hydrolase family 8 and adopts a classical (α/α)-barrel fold. This architecture forms a deep groove which may serve as the substrate-binding pocket. The conserved catalytic residues (Glu72, Trp110, Asn119, Phe167, Tyr183 and Asp232) are localized at the centre of the groove. This crystal structure will help to improve the understanding of the hydrolytic mechanism of PssZ and its application as a biofilm disrupter.

PubMed: 31282870
DOI: 10.1107/S2053230X19008100

Experimental method

X-RAY DIFFRACTION (1.617 Å)