6R2M

Crystal structure of PssZ from Listeria monocytogenes

6R2M の概要

• エントリーDOI: 10.2210/pdb6r2m/pdb
• 分子名称: Glycoside transferase (2 entities in total)
• 機能のキーワード: glycosylhydrolase, exopolysacharide, monomer, extracellular, hydrolase
• 由来する生物種: Listeria monocytogenes
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 75684.35

構造登録者

Wu, H.,Cheng, J.,Qiao, S.,Li, D.,Ma, L. (登録日: 2019-03-18, 公開日: 2019-07-24, 最終更新日: 2024-05-15)

主引用文献

Wu, H.,Qiao, S.,Li, D.,Guo, L.,Zhu, M.,Ma, L.Z.
Crystal structure of the glycoside hydrolase PssZ from Listeria monocytogenes.
Acta Crystallogr.,Sect.F, 75:501-506, 2019

PubMed Abstract: Biofilms are microbial communities that are embedded in the extracellular matrix. The exopolysaccharide (EPS) is a key component of the biofilm matrix that maintains the structure of the biofilm and protects the bacteria from antimicrobials. Microbial glycoside hydrolases have been exploited to disrupt biofilms by breaking down EPSs. PssZ has recently been identified as a glycoside hydrolase that can disperse aggregates of Listeria monocytogenes. In this study, the crystal structure of PssZ has been determined at 1.6 Å resolution. PssZ belongs to glycoside hydrolase family 8 and adopts a classical (α/α)-barrel fold. This architecture forms a deep groove which may serve as the substrate-binding pocket. The conserved catalytic residues (Glu72, Trp110, Asn119, Phe167, Tyr183 and Asp232) are localized at the centre of the groove. This crystal structure will help to improve the understanding of the hydrolytic mechanism of PssZ and its application as a biofilm disrupter.

PubMed: 31282870
DOI: 10.1107/S2053230X19008100

実験手法

X-RAY DIFFRACTION (1.617 Å)