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6R2I

Crystal structure of the SUN1-KASH5 6:6 complex

Summary for 6R2I
Entry DOI10.2210/pdb6r2i/pdb
Related6R15 6R16
DescriptorSUN domain-containing protein 1, KASH5, POTASSIUM ION, ... (4 entities in total)
Functional Keywordssun1, kash5, linc complex, nucleoskeleton, cytoskeleton, nuclear envelope, structural protein
Biological sourceHomo sapiens (Human)
More
Total number of polymer chains2
Total formula weight25906.21
Authors
Gurusaran, M.,Davies, O.R. (deposition date: 2019-03-18, release date: 2020-07-15, Last modification date: 2024-01-24)
Primary citationGurusaran, M.,Davies, O.R.
A molecular mechanism for LINC complex branching by structurally diverse SUN-KASH 6:6 assemblies.
Elife, 10:-, 2021
Cited by
PubMed Abstract: The Linker of Nucleoskeleton and Cytoskeleton (LINC) complex mechanically couples cytoskeletal and nuclear components across the nuclear envelope to fulfil a myriad of cellular functions, including nuclear shape and positioning, hearing, and meiotic chromosome movements. The canonical model is that 3:3 interactions between SUN and KASH proteins underlie the nucleocytoskeletal linkages provided by the LINC complex. Here, we provide crystallographic and biophysical evidence that SUN-KASH is a constitutive 6:6 complex in which two constituent 3:3 complexes interact head-to-head. A common SUN-KASH topology is achieved through structurally diverse 6:6 interaction mechanisms by distinct KASH proteins, including zinc-coordination by Nesprin-4. The SUN-KASH 6:6 interface provides a molecular mechanism for the establishment of integrative and distributive connections between 3:3 structures within a branched LINC complex network. In this model, SUN-KASH 6:6 complexes act as nodes for force distribution and integration between adjacent SUN and KASH molecules, enabling the coordinated transduction of large forces across the nuclear envelope.
PubMed: 33393904
DOI: 10.7554/eLife.60175
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.541 Å)
Structure validation

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数据于2025-07-23公开中

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