6R1H

Crystal structure of the LRR ectodomain of the receptor kinase SOBIR1 from Arabidopsis thaliana.

Summary for 6R1H

• Entry DOI: 10.2210/pdb6r1h/pdb
• Descriptor: Leucine-rich repeat receptor-like serine/threonine/tyrosine-protein kinase SOBIR1, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, CHLORIDE ION, ... (6 entities in total)
• Functional Keywords: receptor kinase, lrr receptor, plant immunity, receptor-like protein interacting kinase, immune system
• Biological source: Arabidopsis thaliana (thale cress)
• Total number of polymer chains: 2
• Total formula weight: 67375.84

Authors

Hohmann, U.,Hothorn, M. (deposition date: 2019-03-14, release date: 2019-05-08, Last modification date: 2024-10-23)

Primary citation

Hohmann, U.,Hothorn, M.
Crystal structure of the leucine-rich repeat ectodomain of the plant immune receptor kinase SOBIR1.
Acta Crystallogr D Struct Biol, 75:488-497, 2019

PubMed Abstract: Plant-unique membrane receptor kinases with leucine-rich repeat (LRR) extracellular domains are key regulators of development and immune responses. Here, the 1.55 Å resolution crystal structure of the immune receptor kinase SOBIR1 from Arabidopsis is presented. The ectodomain structure reveals the presence of five LRRs sandwiched between noncanonical capping domains. The disulfide-bond-stabilized N-terminal cap harbours an unusual β-hairpin structure. The C-terminal cap features a highly positively charged linear motif which was found to be largely disordered in this structure. Size-exclusion chromatography and right-angle light-scattering experiments suggest that SOBIR1 is a monomer in solution. The protruding β-hairpin, a set of highly conserved basic residues at the inner surface of the SOBIR LRR domain and the presence of a genetic missense allele in LRR2 together suggest that the SOBIR1 ectodomain may mediate protein-protein interaction in plant immune signalling.

PubMed: 31063151
DOI: 10.1107/S2059798319005291

Experimental method

X-RAY DIFFRACTION (1.55 Å)