6R16

Crystal structure of the SUN1-KASH4 6:6 complex

6R16 の概要

• エントリーDOI: 10.2210/pdb6r16/pdb
• 関連するPDBエントリー: 6R15
• 分子名称: SUN domain-containing protein 1, Nesprin-4, POTASSIUM ION, ... (6 entities in total)
• 機能のキーワード: sun1 kash4 nesprin-4 syne4 linc complex nucleoskeleton cytoskeleton nuclear envelope, structural protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 155622.43

構造登録者

Gurusaran, M.,Davies, O.R. (登録日: 2019-03-13, 公開日: 2020-07-15, 最終更新日: 2024-11-20)

主引用文献

Gurusaran, M.,Davies, O.R.
A molecular mechanism for LINC complex branching by structurally diverse SUN-KASH 6:6 assemblies.
Elife, 10:-, 2021

PubMed Abstract: The Linker of Nucleoskeleton and Cytoskeleton (LINC) complex mechanically couples cytoskeletal and nuclear components across the nuclear envelope to fulfil a myriad of cellular functions, including nuclear shape and positioning, hearing, and meiotic chromosome movements. The canonical model is that 3:3 interactions between SUN and KASH proteins underlie the nucleocytoskeletal linkages provided by the LINC complex. Here, we provide crystallographic and biophysical evidence that SUN-KASH is a constitutive 6:6 complex in which two constituent 3:3 complexes interact head-to-head. A common SUN-KASH topology is achieved through structurally diverse 6:6 interaction mechanisms by distinct KASH proteins, including zinc-coordination by Nesprin-4. The SUN-KASH 6:6 interface provides a molecular mechanism for the establishment of integrative and distributive connections between 3:3 structures within a branched LINC complex network. In this model, SUN-KASH 6:6 complexes act as nodes for force distribution and integration between adjacent SUN and KASH molecules, enabling the coordinated transduction of large forces across the nuclear envelope.

PubMed: 33393904
DOI: 10.7554/eLife.60175

実験手法

X-RAY DIFFRACTION (2.75 Å)