6R15

Crystal structure of the SUN1-KASH1 6:6 complex

Summary for 6R15

• Entry DOI: 10.2210/pdb6r15/pdb
• Descriptor: SUN domain-containing protein 1, Nesprin-1, POTASSIUM ION, ... (6 entities in total)
• Functional Keywords: sun1 kash1 nesprin-1 syne1 linc complex nucleoskeleton cytoskeleton nuclear envelope, structural protein
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 2
• Total formula weight: 26563.96

Authors

Gurusaran, M.,Davies, O.R. (deposition date: 2019-03-13, release date: 2020-04-01, Last modification date: 2024-11-13)

Primary citation

Gurusaran, M.,Davies, O.R.
A molecular mechanism for LINC complex branching by structurally diverse SUN-KASH 6:6 assemblies.
Elife, 10:-, 2021

PubMed Abstract: The Linker of Nucleoskeleton and Cytoskeleton (LINC) complex mechanically couples cytoskeletal and nuclear components across the nuclear envelope to fulfil a myriad of cellular functions, including nuclear shape and positioning, hearing, and meiotic chromosome movements. The canonical model is that 3:3 interactions between SUN and KASH proteins underlie the nucleocytoskeletal linkages provided by the LINC complex. Here, we provide crystallographic and biophysical evidence that SUN-KASH is a constitutive 6:6 complex in which two constituent 3:3 complexes interact head-to-head. A common SUN-KASH topology is achieved through structurally diverse 6:6 interaction mechanisms by distinct KASH proteins, including zinc-coordination by Nesprin-4. The SUN-KASH 6:6 interface provides a molecular mechanism for the establishment of integrative and distributive connections between 3:3 structures within a branched LINC complex network. In this model, SUN-KASH 6:6 complexes act as nodes for force distribution and integration between adjacent SUN and KASH molecules, enabling the coordinated transduction of large forces across the nuclear envelope.

PubMed: 33393904
DOI: 10.7554/eLife.60175

Experimental method

X-RAY DIFFRACTION (1.82 Å)