6QXV

Pink beam serial crystallography: Proteinase K, 1 us exposure, 1585 patterns merged (2 chips)

6QXV の概要

• エントリーDOI: 10.2210/pdb6qxv/pdb
• 分子名称: Proteinase K, CALCIUM ION, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: pink beam, fixed-target, serial crystallography, proteinase k, hydrolase
• 由来する生物種: Parengyodontium album (Tritirachium album)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30202.00

構造登録者

Tolstikova, A.,Oberthuer, D.,Meents, A. (登録日: 2019-03-08, 公開日: 2019-09-25, 最終更新日: 2024-11-06)

主引用文献

Tolstikova, A.,Levantino, M.,Yefanov, O.,Hennicke, V.,Fischer, P.,Meyer, J.,Mozzanica, A.,Redford, S.,Crosas, E.,Opara, N.L.,Barthelmess, M.,Lieske, J.,Oberthuer, D.,Wator, E.,Mohacsi, I.,Wulff, M.,Schmitt, B.,Chapman, H.N.,Meents, A.
1 kHz fixed-target serial crystallography using a multilayer monochromator and an integrating pixel detector.
Iucrj, 6:927-937, 2019

PubMed Abstract: Reliable sample delivery and efficient use of limited beam time have remained bottlenecks for serial crystallography (SX). Using a high-intensity polychromatic X-ray beam in combination with a newly developed charge-integrating JUNGFRAU detector, we have applied the method of fixed-target SX to collect data at a rate of 1 kHz at a synchrotron-radiation facility. According to our data analysis for the given experimental conditions, only about 3 000 diffraction patterns are required for a high-quality diffraction dataset. With indexing rates of up to 25%, recording of such a dataset takes less than 30 s.

PubMed: 31576225
DOI: 10.1107/S205225251900914X

実験手法

X-RAY DIFFRACTION (1.94 Å)