6QS9

Bovine Serum Albumin in complex with Ketoprofen

6QS9 の概要

• エントリーDOI: 10.2210/pdb6qs9/pdb
• 分子名称: Serum albumin, (R)-Ketoprofen, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: non-steroidal anti-inflammatory drug, plasma protein, transport protein
• 由来する生物種: Bos taurus (Cattle)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 139461.67

構造登録者

Donini, S.,Parisini, E. (登録日: 2019-02-20, 公開日: 2019-09-11, 最終更新日: 2024-10-16)

主引用文献

Castagna, R.,Donini, S.,Colnago, P.,Serafini, A.,Parisini, E.,Bertarelli, C.
Biohybrid Electrospun Membrane for the Filtration of Ketoprofen Drug from Water.
Acs Omega, 4:13270-13278, 2019

PubMed Abstract: A current challenge in materials science and biotechnology is to express a specific and controlled functionality on the large interfacial area of a nanostructured material to create smart biohybrid systems for targeted applications. Here, we report on a biohybrid system featuring poly(vinyl alcohol) as the supporting synthetic polymer and bovine serum albumin as the biofunctional element. The optimal processing conditions to produce these self-standing composite membranes are determined, and the composition and distribution of the bioactive agent within the polymeric matrices are analyzed. A post-processing cross-linking using glutaraldehyde enables this functional membrane to be used as a chemical filter in aqueous environments. By demonstrating that our mats can remove large amounts of ketoprofen from water, we show that the combination of a BSA-induced biofunctionality with a nanostructured fibrous material allows for the development of an efficient biohybrid filtering device for the large and widely used family of nonsteroidal anti-inflammatory drugs (NSAIDs). The crystal structure of the complex between BSA and ketoprofen is determined for the first time and confirms the interaction between the two species.

PubMed: 31460455
DOI: 10.1021/acsomega.9b01442

実験手法

X-RAY DIFFRACTION (2.802 Å)