6QQ3

The room temperature structure of lysozyme via the acoustic levitation of a droplet

6QQ3 の概要

• エントリーDOI: 10.2210/pdb6qq3/pdb
• 分子名称: Lysozyme C, SODIUM ION, ACETATE ION, ... (5 entities in total)
• 機能のキーワード: lysozyme hewl hydrolase, acoustic levitation, hydrolase
• 由来する生物種: Gallus gallus (Chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14540.61

構造登録者

Axford, D.N.,Docker, P.,Dye, E.,Morris, R. (登録日: 2019-02-17, 公開日: 2019-03-06, 最終更新日: 2024-10-23)

主引用文献

Morris, R.H.,Dye, E.R.,Axford, D.,Newton, M.I.,Beale, J.H.,Docker, P.T.
Non-Contact Universal Sample Presentation for Room Temperature Macromolecular Crystallography Using Acoustic Levitation.
Sci Rep, 9:12431-12431, 2019

PubMed Abstract: Macromolecular Crystallography is a powerful and valuable technique to assess protein structures. Samples are commonly cryogenically cooled to minimise radiation damage effects from the X-ray beam, but low temperatures hinder normal protein functions and this procedure can introduce structural artefacts. Previous experiments utilising acoustic levitation for beamline science have focused on Langevin horns which deliver significant power to the confined droplet and are complex to set up accurately. In this work, the low power, portable TinyLev acoustic levitation system is used in combination with an approach to dispense and contain droplets, free of physical sample support to aid protein crystallography experiments. This method facilitates efficient X-ray data acquisition in ambient conditions compatible with dynamic studies. Levitated samples remain free of interference from fixed sample mounts, receive negligible heating, do not suffer significant evaporation and since the system occupies a small volume, can be readily installed at other light sources.

PubMed: 31455801
DOI: 10.1038/s41598-019-48612-4

実験手法

X-RAY DIFFRACTION (1.53 Å)