6QP7

Drosophila Semaphorin 2a

6QP7 の概要

• エントリーDOI: 10.2210/pdb6qp7/pdb
• 分子名称: Semaphorin-2A, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (10 entities in total)
• 機能のキーワード: axon guidance cue, developmental protein, cell signalling, semaphorin, signalling protein, signaling protein
• 由来する生物種: Drosophila melanogaster (Fruit fly)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 159499.49

構造登録者

Robinson, R.A.,Rozbesky, D.,Harlos, K.,Siebold, C.,Jones, E.Y. (登録日: 2019-02-13, 公開日: 2019-08-21, 最終更新日: 2024-10-16)

主引用文献

Rozbesky, D.,Robinson, R.A.,Jain, V.,Renner, M.,Malinauskas, T.,Harlos, K.,Siebold, C.,Jones, E.Y.
Diversity of oligomerization in Drosophila semaphorins suggests a mechanism of functional fine-tuning.
Nat Commun, 10:3691-3691, 2019

PubMed Abstract: Semaphorin ligands and their plexin receptors are one of the major cell guidance factors that trigger localised changes in the cytoskeleton. Binding of semaphorin homodimer to plexin brings two plexins in close proximity which is a prerequisite for plexin signalling. This model appears to be too simplistic to explain the complexity and functional versatility of these molecules. Here, we determine crystal structures for all members of Drosophila class 1 and 2 semaphorins. Unlike previously reported semaphorin structures, Sema1a, Sema2a and Sema2b show stabilisation of sema domain dimer formation via a disulfide bond. Unexpectedly, our structural and biophysical data show Sema1b is a monomer suggesting that semaphorin function may not be restricted to dimers. We demonstrate that semaphorins can form heterodimers with members of the same semaphorin class. This heterodimerization provides a potential mechanism for cross-talk between different plexins and co-receptors to allow fine-tuning of cell signalling.

PubMed: 31417095
DOI: 10.1038/s41467-019-11683-y

実験手法

X-RAY DIFFRACTION (1.96 Å)