6QNX

Structure of the SA2/SCC1/CTCF complex

「6QNY」から置き換えられました

6QNX の概要

• エントリーDOI: 10.2210/pdb6qnx/pdb
• 分子名称: Cohesin subunit SA-2, 64-kDa C-terminal product, Transcriptional repressor CTCF, ... (4 entities in total)
• 機能のキーワード: cohesin, ctcf, tad, chromatin folding, genome regulation, sa2, gene regulation
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 130890.87

構造登録者

Li, Y.,Muir, K.W.,Panne, D. (登録日: 2019-02-12, 公開日: 2020-01-22, 最終更新日: 2024-10-16)

主引用文献

Li, Y.,Haarhuis, J.H.I.,Sedeno Cacciatore, A.,Oldenkamp, R.,van Ruiten, M.S.,Willems, L.,Teunissen, H.,Muir, K.W.,de Wit, E.,Rowland, B.D.,Panne, D.
The structural basis for cohesin-CTCF-anchored loops.
Nature, 578:472-476, 2020

PubMed Abstract: Cohesin catalyses the folding of the genome into loops that are anchored by CTCF. The molecular mechanism of how cohesin and CTCF structure the 3D genome has remained unclear. Here we show that a segment within the CTCF N terminus interacts with the SA2-SCC1 subunits of human cohesin. We report a crystal structure of SA2-SCC1 in complex with CTCF at a resolution of 2.7 Å, which reveals the molecular basis of the interaction. We demonstrate that this interaction is specifically required for CTCF-anchored loops and contributes to the positioning of cohesin at CTCF binding sites. A similar motif is present in a number of established and newly identified cohesin ligands, including the cohesin release factor WAPL. Our data suggest that CTCF enables the formation of chromatin loops by protecting cohesin against loop release. These results provide fundamental insights into the molecular mechanism that enables the dynamic regulation of chromatin folding by cohesin and CTCF.

PubMed: 31905366
DOI: 10.1038/s41586-019-1910-z

実験手法

X-RAY DIFFRACTION (2.7 Å)