6QLC

The ssDNA-binding RNA polymerase cofactor Drc from Pseudomonas phage LUZ7

6QLC の概要

• エントリーDOI: 10.2210/pdb6qlc/pdb
• 分子名称: ssDNA binding RNA Polymerase cofactor, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: ssdna binding, viral protein
• 由来する生物種: Pseudomonas phage LUZ7
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11656.20

構造登録者

De Zitter, E.,Boon, M.,De Smet, J.,Lavigne, R.,Van Meervelt, L. (登録日: 2019-01-31, 公開日: 2019-10-30, 最終更新日: 2024-05-15)

主引用文献

Boon, M.,De Zitter, E.,De Smet, J.,Wagemans, J.,Voet, M.,Pennemann, F.L.,Schalck, T.,Kuznedelov, K.,Severinov, K.,Van Meervelt, L.,De Maeyer, M.,Lavigne, R.
'Drc', a structurally novel ssDNA-binding transcription regulator of N4-related bacterial viruses.
Nucleic Acids Res., 48:445-459, 2020

PubMed Abstract: Bacterial viruses encode a vast number of ORFan genes that lack similarity to any other known proteins. Here, we present a 2.20 Å crystal structure of N4-related Pseudomonas virus LUZ7 ORFan gp14, and elucidate its function. We demonstrate that gp14, termed here as Drc (ssDNA-binding RNA Polymerase Cofactor), preferentially binds single-stranded DNA, yet contains a structural fold distinct from other ssDNA-binding proteins (SSBs). By comparison with other SSB folds and creation of truncation and amino acid substitution mutants, we provide the first evidence for the binding mechanism of this unique fold. From a biological perspective, Drc interacts with the phage-encoded RNA Polymerase complex (RNAPII), implying a functional role as an SSB required for the transition from early to middle gene transcription during phage infection. Similar to the coliphage N4 gp2 protein, Drc likely binds locally unwound middle promoters and recruits the phage RNA polymerase. However, unlike gp2, Drc does not seem to need an additional cofactor for promoter melting. A comparison among N4-related phage genera highlights the evolutionary diversity of SSB proteins in an otherwise conserved transcription regulation mechanism.

PubMed: 31724707
DOI: 10.1093/nar/gkz1048

実験手法

X-RAY DIFFRACTION (2.2 Å)