6QJN

Crystal Structure of the third PDZ domain of PSD-95 protein D332G mutant: space group I4122

「5OIJ」から置き換えられました

6QJN の概要

• エントリーDOI: 10.2210/pdb6qjn/pdb
• 分子名称: Disks large homolog 4 (2 entities in total)
• 機能のキーワード: pdz domain, signaling protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 22180.91

構造登録者

Camara-Artigas, A. (登録日: 2019-01-24, 公開日: 2019-04-17, 最終更新日: 2024-01-24)

主引用文献

Camara-Artigas, A.,Murciano-Calles, J.,Martinez, J.C.
Conformational changes in the third PDZ domain of the neuronal postsynaptic density protein 95.
Acta Crystallogr D Struct Biol, 75:381-391, 2019

PubMed Abstract: PDZ domains are protein-protein recognition modules that interact with other proteins through short sequences at the carboxyl terminus. These domains are structurally characterized by a conserved fold composed of six β-strands and two α-helices. The third PDZ domain of the neuronal postsynaptic density protein 95 has an additional α-helix (α3), the role of which is not well known. In previous structures, a succinimide was identified in the β2-β3 loop instead of Asp332. The presence of this modified residue results in conformational changes in α3. In this work, crystallographic structures of the following have been solved: a truncated form of the third PDZ domain of the neuronal postsynaptic density protein 95 from which α3 has been removed, D332P and D332G variants of the protein, and a new crystal form of this domain showing the binding of Asp332 to the carboxylate-binding site of a symmetry-related molecule. Crystals of the wild type and variants were obtained in different space groups, which reflects the conformational plasticity of the domain. Indeed, the overall analysis of these structures suggests that the conformation of the β2-β3 loop is correlated with the fold acquired by α3. The alternate conformation of the β2-β3 loop affects the electrostatics of the carboxylate-binding site and might modulate the binding of different PDZ-binding motifs.

PubMed: 30988255
DOI: 10.1107/S2059798319001980

実験手法

X-RAY DIFFRACTION (1.8 Å)