6QJ4

Crystal structure of the C. thermophilum condensin Ycs4-Brn1 subcomplex bound to the Smc4 ATPase head in complex with the C-terminal domain of Brn1

6QJ4 の概要

• エントリーDOI: 10.2210/pdb6qj4/pdb
• 分子名称: Condensin complex subunit 1,Condensin complex subunit 1,Condensin complex subunit 1, Condensin complex subunit 2, Uncharacterized protein,Uncharacterized protein, ... (5 entities in total)
• 機能のキーワード: condensin, smc complex, atpase, chromosome condensation, loop extrusion, cell cycle
• 由来する生物種: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 212639.78

構造登録者

Hassler, M.,Haering, C.H.,Kschonsak, M. (登録日: 2019-01-22, 公開日: 2019-07-03, 最終更新日: 2024-01-24)

主引用文献

Hassler, M.,Shaltiel, I.A.,Kschonsak, M.,Simon, B.,Merkel, F.,Tharichen, L.,Bailey, H.J.,Macosek, J.,Bravo, S.,Metz, J.,Hennig, J.,Haering, C.H.
Structural Basis of an Asymmetric Condensin ATPase Cycle.
Mol.Cell, 74:1175-1188.e9, 2019

PubMed Abstract: The condensin protein complex plays a key role in the structural organization of genomes. How the ATPase activity of its SMC subunits drives large-scale changes in chromosome topology has remained unknown. Here we reconstruct, at near-atomic resolution, the sequence of events that take place during the condensin ATPase cycle. We show that ATP binding induces a conformational switch in the Smc4 head domain that releases its hitherto undescribed interaction with the Ycs4 HEAT-repeat subunit and promotes its engagement with the Smc2 head into an asymmetric heterodimer. SMC head dimerization subsequently enables nucleotide binding at the second active site and disengages the Brn1 kleisin subunit from the Smc2 coiled coil to open the condensin ring. These large-scale transitions in the condensin architecture lay out a mechanistic path for its ability to extrude DNA helices into large loop structures.

PubMed: 31226277
DOI: 10.1016/j.molcel.2019.03.037

実験手法

X-RAY DIFFRACTION (5.8 Å)