6QFY

CRYSTAL STRUCTURE OF PORCINE HEMAGGLUTINATING ENCEPHALOMYELITIS VIRUS SPIKE PROTEIN LECTIN DOMAIN

6QFY の概要

• エントリーDOI: 10.2210/pdb6qfy/pdb
• 分子名称: Spike glycoprotein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: sialic acid binding, beta-sandwich, viral spike protein, spike-ntd, viral protein
• 由来する生物種: Porcine hemagglutinating encephalomyelitis virus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 68107.54

構造登録者

Huizinga, E.G.,Bakkers, M.,Lang, Y. (登録日: 2019-01-10, 公開日: 2019-02-06, 最終更新日: 2024-01-24)

主引用文献

Hulswit, R.J.G.,Lang, Y.,Bakkers, M.J.G.,Li, W.,Li, Z.,Schouten, A.,Ophorst, B.,van Kuppeveld, F.J.M.,Boons, G.J.,Bosch, B.J.,Huizinga, E.G.,de Groot, R.J.
Human coronaviruses OC43 and HKU1 bind to 9-O-acetylated sialic acids via a conserved receptor-binding site in spike protein domain A.
Proc. Natl. Acad. Sci. U.S.A., 116:2681-2690, 2019

PubMed Abstract: Human betacoronaviruses OC43 and HKU1 are endemic respiratory pathogens and, while related, originated from independent zoonotic introductions. OC43 is in fact a host-range variant of the species , and more closely related to bovine coronavirus (BCoV)-its presumptive ancestor-and porcine hemagglutinating encephalomyelitis virus (PHEV). The β1-coronaviruses (β1CoVs) and HKU1 employ glycan-based receptors carrying 9--acetylated sialic acid (9--Ac-Sia). Receptor binding is mediated by spike protein S, the main determinant of coronavirus host specificity. For BCoV, a crystal structure for the receptor-binding domain S1 is available and for HKU1 a cryoelectron microscopy structure of the complete S ectodomain. However, the location of the receptor-binding site (RBS), arguably the single-most important piece of information, is unknown. Here we solved the 3.0-Å crystal structure of PHEV S1 We then took a comparative structural analysis approach to map the β1CoV S RBS, using the general design of 9--Ac-Sia-binding sites as blueprint, backed-up by automated ligand docking, structure-guided mutagenesis of OC43, BCoV, and PHEV S1, and infectivity assays with BCoV-S-pseudotyped vesicular stomatitis viruses. The RBS is not exclusive to OC43 and related animal viruses, but is apparently conserved and functional also in HKU1 S1 The binding affinity of the HKU1 S RBS toward short sialoglycans is significantly lower than that of OC43, which we attribute to differences in local architecture and accessibility, and which may be indicative for differences between the two viruses in receptor fine-specificity. Our findings challenge reports that would map the OC43 RBS elsewhere in S1 and that of HKU1 in domain S1.

PubMed: 30679277
DOI: 10.1073/pnas.1809667116

実験手法

X-RAY DIFFRACTION (2.97 Å)