6QAZ

Crystal structure of gp41-1 intein

6QAZ の概要

• エントリーDOI: 10.2210/pdb6qaz/pdb
• 分子名称: cyanophage-like gp41-1 intein, CITRATE ANION, PENTAETHYLENE GLYCOL, ... (5 entities in total)
• 機能のキーワード: intein, protein-splicing, metagenomic, unknown function
• 由来する生物種: metagenome
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15230.35

構造登録者

Mikula, K.M.,Beyer, H.M.,Li, M.,Wlodawer, A.,Iwai, H. (登録日: 2018-12-20, 公開日: 2019-11-13, 最終更新日: 2024-01-24)

主引用文献

Beyer, H.M.,Mikula, K.M.,Li, M.,Wlodawer, A.,Iwai, H.
The crystal structure of the naturally split gp41-1 intein guides the engineering of orthogonal split inteins from cis-splicing inteins.
Febs J., 287:1886-1898, 2020

PubMed Abstract: Protein trans-splicing catalyzed by split inteins has increasingly become useful as a protein engineering tool. We solved the 1.0 Å-resolution crystal structure of a fused variant from the naturally split gp41-1 intein, previously identified from environmental metagenomic sequence data. The structure of the 125-residue gp41-1 intein revealed a compact pseudo-C2-symmetry commonly found in the Hedgehog/Intein superfamily with extensive charge-charge interactions between the split N- and C-terminal intein fragments that are common among naturally occurring split inteins. We successfully created orthogonal split inteins by engineering a similar charge network into the same region of a cis-splicing intein. This strategy could be applicable for creating novel natural-like split inteins from other, more prevalent cis-splicing inteins. DATABASE: Structural data are available in the RCSB Protein Data Bank under the accession number 6QAZ.

PubMed: 31665813
DOI: 10.1111/febs.15113

実験手法

X-RAY DIFFRACTION (1.02 Å)