6Q4R

High-resolution crystal structure of ERAP1 with bound phosphinic transition-state analogue inhibitor

Summary for 6Q4R

• Entry DOI: 10.2210/pdb6q4r/pdb
• Descriptor: Endoplasmic reticulum aminopeptidase 1,Endoplasmic reticulum aminopeptidase 1, HEXAETHYLENE GLYCOL, TETRAETHYLENE GLYCOL, ... (13 entities in total)
• Functional Keywords: endoplasmic reticulum aminopeptidase 1, erap1, antigen presentation, hydrolase
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 1
• Total formula weight: 108762.05

Authors

Giastas, P.,Neu, M.,Rowland, P.,Stratikos, E. (deposition date: 2018-12-06, release date: 2019-04-10, Last modification date: 2024-10-16)

Primary citation

Giastas, P.,Neu, M.,Rowland, P.,Stratikos, E.
High-Resolution Crystal Structure of Endoplasmic Reticulum Aminopeptidase 1 with Bound Phosphinic Transition-State Analogue Inhibitor.
Acs Med.Chem.Lett., 10:708-713, 2019

PubMed Abstract: Endoplasmic reticulum aminopeptidase 1 (ERAP1) is an intracellular enzyme that helps generate peptides presented by Major Histocompatibility Complex Class I (MHC class I) molecules and is an emerging target for immunotherapy applications. Despite almost two decades of research on ERAP1, lack of high-resolution crystal structures has hampered drug-development efforts. By optimizing the protein construct, we obtained a high-resolution (1.60 Å) crystal structure of the closed-conformation of ERAP1 with a potent phosphinic pseudopeptide inhibitor bound in its active site. The structure provides key insight on the mechanism of inhibition as well as selectivity toward homologous enzymes and allows detailed mapping of the internal cavity of the enzyme that accommodates peptide-substrates. Bis-tris propane and malic acid molecules, found bound in pockets in the internal cavity, reveal potential druggable secondary binding sites. The ability to obtain high-resolution crystal structures of ERAP1 removes a major bottleneck in the development of compounds that regulate its activity and will greatly accelerate drug-discovery efforts.

PubMed: 31097987
DOI: 10.1021/acsmedchemlett.9b00002

Experimental method

X-RAY DIFFRACTION (1.6 Å)