6Q1M
Crystal structure of the wheat dwarf virus Rep domain
Summary for 6Q1M
| Entry DOI | 10.2210/pdb6q1m/pdb |
| Descriptor | Replication-associated protein, GLYCEROL (3 entities in total) |
| Functional Keywords | huh-tag, huh motif, rep domain, viral protein, single stranded dna, ssdna, ssdna binding, replication |
| Biological source | Wheat dwarf virus |
| Total number of polymer chains | 1 |
| Total formula weight | 15978.92 |
| Authors | Litzau, L.A.,Everett, B.A.,Evans III, R.L.,Shi, K.,Tompkins, K.,Gordon, W.R. (deposition date: 2019-08-05, release date: 2019-12-11, Last modification date: 2023-10-11) |
| Primary citation | Everett, B.A.,Litzau, L.A.,Tompkins, K.,Shi, K.,Nelson, A.,Aihara, H.,Evans Iii, R.L.,Gordon, W.R. Crystal structure of the Wheat dwarf virus Rep domain. Acta Crystallogr.,Sect.F, 75:744-749, 2019 Cited by PubMed Abstract: The Rep domain of Wheat dwarf virus (WDV Rep) is an HUH endonuclease involved in rolling-circle replication. HUH endonucleases coordinate a metal ion to enable the nicking of a specific ssDNA sequence and the subsequent formation of an intermediate phosphotyrosine bond. This covalent protein-ssDNA adduct makes HUH endonucleases attractive fusion tags (HUH-tags) in a diverse number of biotechnological applications. Solving the structure of an HUH endonuclease in complex with ssDNA will provide critical information about ssDNA recognition and sequence specificity, thus enabling rationally engineered protein-DNA interactions that are programmable. The structure of the WDV Rep domain reported here was solved in the apo state from a crystal diffracting to 1.24 Å resolution and represents an initial step in the direction of solving the structure of a protein-ssDNA complex. PubMed: 31797816DOI: 10.1107/S2053230X19015796 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.24 Å) |
Structure validation
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