6PXK

3.65 Angstroms resolution structure of HslU with an axial-channel plug

6PXK の概要

• エントリーDOI: 10.2210/pdb6pxk/pdb
• 分子名称: ATP-dependent protease ATPase subunit HslU, unidentified alpha helical sequence, ADENOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: aaa+ atpase, peptidase, hydrolase
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 13
• 化学式量合計: 619647.26

構造登録者

Baytshtok, V.,Grant, R.A.,Sauer, R.T. (登録日: 2019-07-26, 公開日: 2020-07-29, 最終更新日: 2024-11-06)

主引用文献

Baytshtok, V.,Fei, X.,Shih, T.T.,Grant, R.A.,Santos, J.C.,Baker, T.A.,Sauer, R.T.
Heat activates the AAA+ HslUV protease by melting an axial autoinhibitory plug.
Cell Rep, 34:108639-108639, 2021

PubMed Abstract: At low temperatures, protein degradation by the AAA+ HslUV protease is very slow. New crystal structures reveal that residues in the intermediate domain of the HslU unfoldase can plug its axial channel, blocking productive substrate binding and subsequent unfolding, translocation, and degradation by the HslV peptidase. Biochemical experiments with wild-type and mutant enzymes support a model in which heat-induced melting of this autoinhibitory plug activates HslUV proteolysis.

PubMed: 33472065
DOI: 10.1016/j.celrep.2020.108639

実験手法

X-RAY DIFFRACTION (3.647 Å)