6PTO

Structure of Ctf4 trimer in complex with three CMG helicases

これはPDB形式変換不可エントリーです。

6PTO の概要

• エントリーDOI: 10.2210/pdb6pto/pdb
• EMDBエントリー: 20473
• 分子名称: DNA polymerase alpha-binding protein, Minichromosome maintenance protein 5, DNA replication licensing factor MCM6, ... (13 entities in total)
• 機能のキーワード: dna replication, cryo-em, cmg-ctf4, replication
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 36
• 化学式量合計: 2674619.90

構造登録者

Yuan, Z.,Georgescu, R.,Bai, L.,Santos, R.,Donnell, M.,Li, H. (登録日: 2019-07-16, 公開日: 2019-11-20, 最終更新日: 2024-10-16)

主引用文献

Yuan, Z.,Georgescu, R.,Santos, R.L.A.,Zhang, D.,Bai, L.,Yao, N.Y.,Zhao, G.,O'Donnell, M.E.,Li, H.
Ctf4 organizes sister replisomes and Pol alpha into a replication factory.
Elife, 8:-, 2019

PubMed Abstract: The current view is that eukaryotic replisomes are independent. Here we show that Ctf4 tightly dimerizes CMG helicase, with an extensive interface involving Psf2, Cdc45, and Sld5. Interestingly, Ctf4 binds only one Pol α-primase. Thus, Ctf4 may have evolved as a trimer to organize two helicases and one Pol α-primase into a replication factory. In the 2CMG-Ctf4-1Pol α-primase factory model, the two CMGs nearly face each other, placing the two lagging strands toward the center and two leading strands out the sides. The single Pol α-primase is centrally located and may prime both sister replisomes. The Ctf4-coupled-sister replisome model is consistent with cellular microscopy studies revealing two sister forks of an origin remain attached and are pushed forward from a protein platform. The replication factory model may facilitate parental nucleosome transfer during replication.

PubMed: 31589141
DOI: 10.7554/eLife.47405

実験手法

ELECTRON MICROSCOPY (7 Å)