6PRM

Crystal structure of apo PsS1_19B

6PRM の概要

• エントリーDOI: 10.2210/pdb6prm/pdb
• 分子名称: exo-4S-kappa carrageenan S1 sulfatase, CALCIUM ION (3 entities in total)
• 機能のキーワード: s1 sulfatase, hydrolase
• 由来する生物種: Pseudoalteromonas fuliginea
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 212430.92

構造登録者

Hettle, A.G.,Boraston, A.B. (登録日: 2019-07-10, 公開日: 2019-09-25, 最終更新日: 2023-10-11)

主引用文献

Hettle, A.G.,Hobbs, J.K.,Pluvinage, B.,Vickers, C.,Abe, K.T.,Salama-Alber, O.,McGuire, B.E.,Hehemann, J.H.,Hui, J.P.M.,Berrue, F.,Banskota, A.,Zhang, J.,Bottos, E.M.,Van Hamme, J.,Boraston, A.B.
Insights into the kappa / iota-carrageenan metabolism pathway of some marinePseudoalteromonasspecies.
Commun Biol, 2:474-474, 2019

PubMed Abstract: is a globally distributed marine-associated genus that can be found in a broad range of aquatic environments, including in association with macroalgal surfaces where they may take advantage of these rich sources of polysaccharides. The metabolic systems that confer the ability to metabolize this abundant form of photosynthetically fixed carbon, however, are not yet fully understood. Through genomics, transcriptomics, microbiology, and specific structure-function studies of pathway components we address the capacity of newly isolated marine pseudoalteromonads to metabolize the red algal galactan carrageenan. The results reveal that the κ/ι-carrageenan specific polysaccharide utilization locus (CarPUL) enables isolates possessing this locus the ability to grow on this substrate. Biochemical and structural analysis of the enzymatic components of the CarPUL promoted the development of a detailed model of the κ/ι-carrageenan metabolic pathway deployed by pseudoalteromonads, thus furthering our understanding of how these microbes have adapted to a unique environmental niche.

PubMed: 31886414
DOI: 10.1038/s42003-019-0721-y

実験手法

X-RAY DIFFRACTION (2.5 Å)