6PQU

Cryo-EM structure of HzTransib/nicked TIR substrate DNA pre-reaction complex (PRC)

6PQU の概要

• エントリーDOI: 10.2210/pdb6pqu/pdb
• EMDBエントリー: 20452 20453
• 分子名称: DNA-mediated transposase, DNA (5'-D(P*AP*TP*CP*TP*GP*GP*CP*CP*TP*AP*GP*AP*TP*CP*T)-3'), DNA (5'-D(P*CP*AP*CP*GP*GP*TP*GP*GP*AP*TP*CP*GP*AP*AP*AP*A)-3'), ... (7 entities in total)
• 機能のキーワード: rag-like transposase, dde family enzyme, transib, terminal inverted repeat., recombination, recombination-dna complex, recombination/dna
• 由来する生物種: Helicoverpa zea (Corn earworm moth); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 152698.54

構造登録者

Liu, C.,Yang, Y.,Schatz, D.G. (登録日: 2019-07-10, 公開日: 2019-10-09, 最終更新日: 2024-03-20)

主引用文献

Liu, C.,Yang, Y.,Schatz, D.G.
Structures of a RAG-like transposase during cut-and-paste transposition.
Nature, 575:540-544, 2019

PubMed Abstract: Transposons have had a pivotal role in genome evolution and are believed to be the evolutionary progenitors of the RAG1-RAG2 recombinase, an essential component of the adaptive immune system in jawed vertebrates. Here we report one crystal structure and five cryo-electron microscopy structures of Transib, a RAG1-like transposase from Helicoverpa zea, that capture the entire transposition process from the apo enzyme to the terminal strand transfer complex with transposon ends covalently joined to target DNA, at resolutions of 3.0-4.6 Å. These structures reveal a butterfly-shaped complex that undergoes two cycles of marked conformational changes in which the 'wings' of the transposase unfurl to bind substrate DNA, close to execute cleavage, open to release the flanking DNA and close again to capture and attack target DNA. Transib possesses unique structural elements that compensate for the absence of a RAG2 partner, including a loop that interacts with the transposition target site and an accordion-like C-terminal tail that elongates and contracts to help to control the opening and closing of the enzyme and assembly of the active site. Our findings reveal the detailed reaction pathway of a eukaryotic cut-and-paste transposase and illuminate some of the earliest steps in the evolution of the RAG recombinase.

PubMed: 31723264
DOI: 10.1038/s41586-019-1753-7

実験手法

ELECTRON MICROSCOPY (3.3 Å)