6PQ0

LCP-embedded Proteinase K treated with MPD

6PQ0 の概要

• エントリーDOI: 10.2210/pdb6pq0/pdb
• 分子名称: Proteinase K, CALCIUM ION (3 entities in total)
• 機能のキーワード: proteinase k, lcp, mpd, microed, hydrolase
• 由来する生物種: Parengyodontium album (Engyodontium album, Tritirachium album)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29010.94

構造登録者

Bu, G.,Zhu, L.,Jing, L.,Shi, D.,Gonen, T.,Liu, W.,Nannenga, B.L. (登録日: 2019-07-08, 公開日: 2020-08-05, 最終更新日: 2024-10-30)

主引用文献

Zhu, L.,Bu, G.,Jing, L.,Shi, D.,Lee, M.Y.,Gonen, T.,Liu, W.,Nannenga, B.L.
Structure Determination from Lipidic Cubic Phase Embedded Microcrystals by MicroED.
Structure, 28:1149-1159.e4, 2020

PubMed Abstract: The lipidic cubic phase (LCP) technique has proved to facilitate the growth of high-quality crystals that are otherwise difficult to grow by other methods. However, the crystal size optimization process could be time and resource consuming, if it ever happens. Therefore, improved techniques for structure determination using these small crystals is an important strategy in diffraction technology development. Microcrystal electron diffraction (MicroED) is a technique that uses a cryo-transmission electron microscopy to collect electron diffraction data and determine high-resolution structures from very thin micro- and nanocrystals. In this work, we have used modified LCP and MicroED protocols to analyze crystals embedded in LCP converted by 2-methyl-2,4-pentanediol or lipase, including Proteinase K crystals grown in solution, cholesterol crystals, and human adenosine A receptor crystals grown in LCP. These results set the stage for the use of MicroED to analyze microcrystalline samples grown in LCP, especially for those highly challenging membrane protein targets.

PubMed: 32735770
DOI: 10.1016/j.str.2020.07.006

実験手法

ELECTRON CRYSTALLOGRAPHY (2 Å)