6PMP

Crystal structure of a fragment of rat phospholipase Cepsilon EF3-RA1

Summary for 6PMP

• Entry DOI: 10.2210/pdb6pmp/pdb
• Descriptor: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1, CALCIUM ION (3 entities in total)
• Functional Keywords: phospholipase, lipase, tim barrel, ras-association domain, signaling protein
• Biological source: Rattus norvegicus (Rat)
• Total number of polymer chains: 4
• Total formula weight: 370438.97

Authors

Rugema, N.Y.,Lyon, A.M. (deposition date: 2019-07-02, release date: 2020-07-08, Last modification date: 2023-10-11)

Primary citation

Rugema, N.Y.,Garland-Kuntz, E.E.,Sieng, M.,Muralidharan, K.,Van Camp, M.M.,O'Neill, H.,Mbongo, W.,Selvia, A.F.,Marti, A.T.,Everly, A.,McKenzie, E.,Lyon, A.M.
Structure of phospholipase C epsilon reveals an integrated RA1 domain and previously unidentified regulatory elements.
Commun Biol, 3:445-445, 2020

PubMed Abstract: Phospholipase Cε (PLCε) generates lipid-derived second messengers at the plasma and perinuclear membranes in the cardiovascular system. It is activated in response to a wide variety of signals, such as those conveyed by Rap1A and Ras, through a mechanism that involves its C-terminal Ras association (RA) domains (RA1 and RA2). However, the complexity and size of PLCε has hindered its structural and functional analysis. Herein, we report the 2.7 Å crystal structure of the minimal fragment of PLCε that retains basal activity. This structure includes the RA1 domain, which forms extensive interactions with other core domains. A conserved amphipathic helix in the autoregulatory X-Y linker of PLCε is also revealed, which we show modulates activity in vitro and in cells. The studies provide the structural framework for the core of this critical cardiovascular enzyme that will allow for a better understanding of its regulation and roles in disease.

PubMed: 32796910
DOI: 10.1038/s42003-020-01178-8

Experimental method

X-RAY DIFFRACTION (2.73 Å)